Publications

Targeted genetic and small molecule disruption of N-Ras CaaX cleavage alters its localization and oncogenic potential.
Emily R Hildebrandt, Shaneela A Hussain, Michelle A Sieburg, Rajani Ravishankar, Nadeem Asad, Sangram Gore, Takahiro Ito, James L Hougland, Timothy M Dore, Walter K Schmidt.
Bioorg Chem. 2024 Jun:147:107316. doi: 10.1016/j.bioorg.2024.107316. Epub 2024 Mar 27. PMID: 38583246.
Library Screening, In Vivo Confirmation, and Structural and Bioinformatic Analysis of Pentapeptide Sequences as Substrates for Protein Farnesyltransferase.
Garrett L Schey, Emily R Hildebrandt, You Wang, Safwan Diwan, Holly A Passetti, Gavin W Potts, Andrea M Sprague-Getsy, Ethan R Leoni, Taylor S Kuebler, Yuk Y Sham, James L Hougland, Lorena S Beese, Walter K Schmidt, Mark D Distefano .
Int J Mol Sci. 2024 May 13;25(10):5324. doi: 10.3390/ijms25105324. PMID: 38791363.
Combined Computational-Biochemical Approach Offers an Accelerated Path to Membrane Protein Solubilization.
Mariah R Pierce, Jingjing Ji, Sadie X Novak, Michelle A Sieburg, Shivangi Nangia, Shikha Nangia, James L Hougland.
J Chem Inf Model. 2023 Nov 27;63(22):7159-7170. doi: 10.1021/acs.jcim.3c00917. Epub 2023 Nov 8. PMID: 37939203.
Synthesis, Enzymatic Peptide Incorporation, and Applications of Diazirine-Containing Isoprenoid Diphosphate Analogues.
Katarzyna Justyna, Riki Das, Ellen L Lorimer, Jiayue Hu, Jodi S Pedersen, Andrea M Sprague-Getsy, Garrett L Schey, Michelle A Sieburg, Olivia J Koehn, Yen-Chih Wang, Yong-Xiang Chen, James L Hougland, Carol L Williams, Mark D Distefano.
Org Lett. 2023 Sep 15;25(36):6767-6772. doi: 10.1021/acs.orglett.3c02736. Epub 2023 Sep 5. PMID: 37669435.
Cellular Uptake of a Fluorescent Ligand Reveals Ghrelin O-Acyltransferase Interacts with Extracellular Peptides and Exhibits Unexpected Localization for a Secretory Pathway Enzyme.
Maria B Campaña, Tasha R Davis, Sadie X Novak, Elizabeth R Cleverdon, Michael Bates, Nikhila Krishnan, Erin R Curtis, Marina D Childs, Mariah R Pierce, Yasandra Morales-Rodriguez, Michelle A Sieburg, Heidi Hehnly, Leonard G Luyt, James L Hougland.
ACS Chem Biol. 2023 Jul 26. doi: 10.1021/acschembio.3c00334. Online ahead of print. PMID: 37494676.
A rising tide lifts all MBOATs: recent progress in structural and functional understanding of membrane bound O-acyltransferases.
Mariah R Pierce and James L Hougland.
Front Physiol. 2023 May 4;14:1167873. doi: 10.3389/fphys.2023.1167873. eCollection 2023. PMID: 37250116.
Initial pharmacological characterization of a major hydroxy metabolite of PF-5190457: inverse agonist activity of PF-6870961 at the ghrelin receptor.
Deschaine SL, Hedegaard MA, Pince CL, Farokhnia M, Moose JE, Stock IA, Adusumalli S, Akhlaghi F, Hougland JL, Sulima A, Rice KC, Koob GF, Vendruscolo L, Holst B, Leggio L.
J Pharmacol Exp Ther. 2023 Jan 11:JPET-AR-2022-001393. doi: 10.1124/jpet.122.001393. Online ahead of print. PMID: 36631279.
Photoswitchable Isoprenoid Lipids Enable Optical Control of Peptide Lipidation.
Morstein J, Bader T, Cardillo AL, Schackmann J, Ashok S, Hougland JL, Hrycyna CA, Trauner DH, Distefano MD.
ACS Chem Biol. 2022 Oct 21;17(10):2945-2953. doi: 10.1021/acschembio.2c00645. Epub 2022 Oct 4. PMID: 36194691.
Temperature-Responsive Nano-Biomaterials from Genetically Encoded Farnesylated Disordered Proteins.
Hossain MS, Zhang Z, Ashok S, Jenks AR, Lynch CJ, Hougland JL, Mozhdehi D.
ACS Appl Bio Mater. 2022 May 16;5(5):1846-1856. doi: 10.1021/acsabm.1c01162. Epub 2022 Jan 19.
PMID: 35044146 Review.
A closer look at alcohol-induced changes in the ghrelin system: novel insights from preclinical and clinical data.
Deschaine SL, Farokhnia M, Gregory-Flores A, Zallar LJ, You ZB, Sun H, Harvey DM, Marchette RCN, Tunstall BJ, Mani BK, Moose JE, Lee MR, Gardner E, Akhlaghi F, Roberto M, Hougland JL, Zigman JM, Koob GF, Vendruscolo LF, Leggio L.
Addict Biol. 2022 Jan;27(1):e13033. doi: 10.1111/adb.13033. Epub 2021 Apr 27. PMID: 33908131 Clinical Trial.
MALDI-MS Analysis of Peptide Libraries Expands the Scope of Substrates for Farnesyltransferase.
Schey GL, Buttery PH, Hildebrandt ER, Novak SX, Schmidt WK, Hougland JL, Distefano MD.
Int J Mol Sci. 2021 Nov 7;22(21):12042. doi: 10.3390/ijms222112042. PMID: 34769472.
Ghrelin octanoylation by ghrelin O-acyltransferase: protein acylation impacting metabolic and neuroendocrine signalling.
Davis TR, Pierce MR, Novak SX, Hougland JL.
Open Biol. 2021 Jul;11(7):210080. doi: 10.1098/rsob.210080. Epub 2021 Jul 28. PMID: 34315274 Review.
Engineering reversible cell-cell interactions using enzymatically lipidated chemically self-assembled nanorings.
Wang Y, Kilic O, Csizmar CM, Ashok S, Hougland JL, Distefano MD, Wagner CR.
Chem Sci. 2020 Oct 26;12(1):331-340. doi: 10.1039/d0sc03194a. PMID: 34168743.
An overview of ghrelin O-acyltransferase inhibitors: a literature and patent review for 2010-2019.
Moose JE, Leets KA, Mate NA, Chisholm JD, Hougland JL.
Expert Opin Ther Pat. 2020 Aug;30(8):581-593. doi: 10.1080/13543776.2020.1776263. Epub 2020 Jun 21.
PMID: 32564644 Review.
Protein Farnesyltransferase Catalyzes Unanticipated Farnesylation and Geranylgeranylation of Shortened Target Sequences.
Ashok S, Hildebrandt ER, Ruiz CS, Hardgrove DS, Coreno DW, Schmidt WK, Hougland JL.
Biochemistry. 2020 Mar 24;59(11):1149-1162. doi: 10.1021/acs.biochem.0c00081. Epub 2020 Mar 10. PMID: 32125828.
Ghrelin Signaling: GOAT and GHS-R1a Take a LEAP in Complexity.
Abizaid A, Hougland JL.
Trends Endocrinol Metab. 2020 Feb;31(2):107-117. doi: 10.1016/j.tem.2019.09.006. Epub 2019 Oct 19. PMID: 31636018.
The ghrelin O-acyltransferase structure reveals a catalytic channel for transmembrane hormone acylation.
Maria B Campaña, Flaviyan Jerome Irudayanathan, Tasha R Davis, Kayleigh R McGovern-Gooch, Rosemary Loftus, Mohammad Ashkar, Najae Escoffery, Melissa Navarro, Michelle A Sieburg, Shikha Nangia, James L Hougland.
J Biol Chem. 2019 Sep 27;294(39):14166-14174. doi: 10.1074/jbc.AC119.009749. Epub 2019 Aug 14. PMID: 31413115.
Ghrelin octanoylation by ghrelin O-acyltransferase: Unique protein biochemistry underlying metabolic signaling.
Hougland JL.
Biochem Soc Trans. 2019 Feb 28;47(1):169-178. doi: 10.1042/BST20180436. Epub 2019 Jan 9. PMID: 30626708 Review.
Biochemical Assays for Ghrelin Acylation and Inhibition of Ghrelin O-Acyltransferase.
Sieburg MA, Cleverdon ER, Hougland JL.
Methods Mol Biol. 2019;2009:227-241. doi: 10.1007/978-1-4939-9532-5_18. PMID: 31152408.

Pubmed